Using IHC to Study Immunocompromised Lymphatic Vessels
Presented by: Gianna Galu
Faculty Advisor: Dr. Steven Burns, Professor of Chemistry & Kimberly Burns, Instructor of Biology
Recent studies have suggested that immunocompromised individuals are susceptible to neurodegenerative diseases, such as Alzheimer’s or Multiple Sclerosis, due to varying conditions of their lymphatic vessels. Immunocompromised individuals often have altered macrophages that make up the microglia in the brain, similar to nearby lymphatic vessels. This experiment is being conducted to determine if immunocompromised individuals not only have altered microglia but also altered lymphatic vessels thus leading to lack of filtration, brain damage, and subsequent neurodegenerative diseases. In order to make this comparison, a specific antibody, LYVE-1, will be used to attach to a specific receptor found on the surface of lymphatic cells in the brain. Since this antibody only binds to a specific receptor on a specific cell, this immunohistochemistry technique is crucial when identifying cell markers. The antibody LYVE-1 will be used to compare the lymphatic vessels of immunocompetent and immunocompromised mouse tissue, with the long-term goal of supporting future human neurodegenerative research.